mouse anti pig cd163 rpe  (Bio-Rad)

Journal: Journal of Animal Science and Biotechnology

Article Title: Heat stress induced testicular impairment is related to orchitis and complement activation in Rongchang boars

doi: 10.1186/s40104-025-01296-5

Figure Lengend Snippet: Testicular immunity and orchitis under HS conditions. A and B GO (A) and KEGG (B) enrichments based on Gene Set Enrichment analysis. C Protein-protein interaction network. D Representative photos of collagen fibers stained by Masson in the testes. E and F Levels of TNF-α (E) and IL-1β (F) in plasma isolated from the spermatic vein. G Representative flow cytometry quantifications of macrophages in the testes. H and I Percentages of CD68 + CD163 + and CD68 + cells among CD45 + cells in the testes. * P < 0.05, ** P < 0.01

Article Snippet: The remaining cells were resuspended in 100 μL Stain Buffer (BD, Franklin Lakes, New Jersey, USA) containing mouse anti-pig CD45: FITC (Bio-Rad, Hercules, California, USA), mouse anti-pig CD163: RPE (Bio-Rad, Hercules, California, USA), and BV421 rat anti-CD11b (BD, Franklin Lakes, New Jersey, USA) at 4 °C for 30 min. Each suspension was centrifuged at 350 × g for 5 min at 4 °C to remove the supernatant.

Techniques: Staining, Clinical Proteomics, Isolation, Flow Cytometry

Journal: Journal of Animal Science and Biotechnology

Article Title: Heat stress induced testicular impairment is related to orchitis and complement activation in Rongchang boars

doi: 10.1186/s40104-025-01296-5

Figure Lengend Snippet: Changes in the TM profile and complement pathway under HS conditions. A tSNE graph of identified cells. B Percentages of cells in each group. C KEGG enrichment of the DEGs from TMs. D Expression levels of C1QA , C1QB , and C1QC in testicular cells as assessed by scRNA-seq. E tSNE graph of TM subclusters in each group. F Percentages of subclusters in each group. G KEGG enrichment of marker genes in Subcluster 5. H Expression levels of C1QA , C1QB , and C1QC in TM subclusters as assessed by scRNA-seq. I KEGG enrichment of DEGs from TM Subcluster 5. J Addmodulescore of subclusters. K tSNE graph of C1QA , C1QB , C1QC , and CD163 as assessed by scRNA-seq

Article Snippet: The remaining cells were resuspended in 100 μL Stain Buffer (BD, Franklin Lakes, New Jersey, USA) containing mouse anti-pig CD45: FITC (Bio-Rad, Hercules, California, USA), mouse anti-pig CD163: RPE (Bio-Rad, Hercules, California, USA), and BV421 rat anti-CD11b (BD, Franklin Lakes, New Jersey, USA) at 4 °C for 30 min. Each suspension was centrifuged at 350 × g for 5 min at 4 °C to remove the supernatant.

Techniques: Expressing, Marker

Journal: Biology Open

Article Title: Heterogeneity and plasticity of porcine alveolar macrophage and pulmonary interstitial macrophage isolated from healthy pigs in vitro

doi: 10.1242/bio.046342

Figure Lengend Snippet: CD163+ rates on PAM cells and IM cells isolated from healthy pigs. Gray area represents the surface CD163 expression levels in PAM cells (A) and IM cells (B) and the white area represents the isotype control. ** P <0.01.

Article Snippet: Surface staining reagents including Mouse anti Pig CD163: RPE (MCA2311PE, Bio-Rad).

Techniques: Isolation, Expressing, Control